Influence of standard and stimulated growth of B16/F10 melanoma on AIF levels in mitochondria in cells of the heart and other somatic organs in female mice
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Aims: studying AIF levels in mitochondria in cells of the heart and various organs in female mice with the growth of experimental melanoma B16 / F10 and comorbid pathology.
Materials and methods
The study included female mice of the С57ВL/6 strain. The experimental groups were composed as follows: an intact group, n=21; animals with chronic neurogenic pain (CNP), n=21; a group with an isolated growth of B16/F10 melanoma, n=63; and a group with B16/F10 melanoma growing in the presence of CNP, n=63. The levels of AIF (ng/mg protein) were determined in mitochondria by ELISA (RayBiotech, USA). Statistical analysis was performed by the Statistica 10.0 software.
CNP upregulated the levels of AIF in mitochondria in the brain by 7.2 times, in the heart by 2.3 times, in the liver by 1.5 times (p<0,001), and in the skin by 1.4 times (p<0,001). The AIF dynamics in mitochondria of the internal organs depended on the stage of the isolated melanoma development, while AIF in the melanoma itself was lower than that in the intact skin: after 1 week by 6.5 times, after 2 weeks by 8.7 times and after 3 weeks by 3.5 times, respectively. The development of melanoma in the presence of CNP was accompanied by a decrease in high AIF levels almost in all internal organs; low AIF levels were recorded in tissues of melanoma growing in the presence of CNP, compared to the levels in the skin of mice with CNP.
Stimulated melanoma growth at the terminal stage is characterized by low AIF levels in the heart and in most organs, the skin and the tumor, which indicates suppression of the electron transfer chain.
Oleg I. Kit, Elena M. Frantsiyants, Irina V.Neskubina, Natalia D. Cheryarina, Alla I. Shikhlyarova, Yuriy V. Przhedetskiy, Viktoriya V. Pozdnyakova, Ekaterina I. Surikova, Irina V. Kaplieva, Valerija A. Bandovkina. Influence of standard and stimulated growth of B16/F10 melanoma on AIF levels in mitochondria in cells of the heart and other somatic organs in female mice. Cardiometry; Issue 18; May 2021; p.113-120; DOI: 10.18137/cardiometry.2021.18.113120; Available from: http://www.cardiometry.net/issues/no18-may-2021/melanoma-on-aif-levels-in-mitochondria