Insulin-like growth factors and their binding proteins in the heart in rats in experimental diabetes mellitus, growing Guerin’s carcinoma and under their combination

Abstract

Diabetes mellitus is an additional risk factor for the development of heart diseases, cardiovascular dysfunction and malignant tumors. The aim of the study was to analyze levels of IGF and IGFBP in heart samples of animals with diabetes mellitus and/or growing Guerin’s carcinoma. The study included white outbred rats of both genders weighing 180-220 g. The rats of each gender were divided into groups of 8 animals: the intact group; test groups 1 (with diabetes) and 2 (with transplanted Guerin’s carcinoma); the main group (transplanted Guerin’s carcinoma growing in the presence of diabetes mellitus). Levels of IGF-I, IGF-II, IGFBP-1, and IGFBP-2 were measured by ELISA (Mediagnost, Germany) in heart homogenates in animals of all groups.

Results

The levels of IGF were not changed in female rats with diabetes and/or Guerin’s carcinoma; however, diabetes mellitus upregulated IGFBP1 and downregulated IGFBP2. The levels of IGF-I, IGF-II and IGFBP2 decreased in males with diabetes mellitus and/or Guerin’s carcinoma, and IGFBP1 decreased only in males with diabetes.

Conclusions

The study has demonstrated that the system of insulin-like growth factors and binding proteins in the heart has a gender specificity, which probably determines the pathogenetic mechanisms of diabetes-induced damage to the heart muscle. The metabolic characteristics typical of this endocrine disease dominate in the IGF-heart system over the changes caused by the growth of a malignant tumor, thereby determining the possible mechanisms for the stability or restoration of the heart function in various pathological conditions.

Imprint

Elena M. Frantsiyants, Valerija A. Bandovkina, Irina V. Kaplieva, Ekaterina I. Surikova, Natalia D. Cheryarina, Alla I. Shikhlyarova, Irina V. Neskubina, Yulia А. Pogorelova, Lidija K. Trepitaki, Irina A. Goroshinskaya, Inga M. Kotieva, Maria I. Morozova. Influence of standard and stimulated growth of B16/F10 melanoma on AIF levels in mitochondria in cells of the heart and other somatic organs in female mice. Cardiometry; Issue 18; May 2021; p.138-144; DOI: 10.18137/cardiometry.2021.18.138144; Available from: http://www.cardiometry.net/issues/no18-may-2021/insulin-like-growth-factors