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Original research

Comparative Studies on the Effect of Tris-Egg Yolk and Soya Milk Based Semen Extender on various Semen Parameters for Effective Long Term Cryopreservation

Abstract

Aim: To analyze the most effective Novel semen extender for long time cryopreservation among Tris egg yolk extender and Soya milk extender. Materials and Methods: The sample size was calculated using clinCalc.com with an alpha error-threshold at 0.05, 95 % confidence interval, G power at 80 %, and enrolment ratio 1. The study was repeated 14 times (N=14) and was measured using statistical Independent Samples Test. The study contained a total sample size of 28 with 2 different groups. Tris egg yolk and soya milk based novel semen extenders were added to the collected semen samples and few drops of the sample was placed on a makler counting chamber which was used for analyzing the concentration, sperm motility and sperm count of the semen samples. Results: The Tris egg yolk based semen extenders were better than the soya milk based semen extenders. Estimated sperm motility and sperm count were found to be P>0.05, revealing statistical insignificance. Conclusion: The sperm parameters were preserved well in Tris egg yolk based semen extender when compared with soya milk based semen extender with respect to sperm count and sperm motility.

Imprint

Reshika Nelsiya N, Vickram A S. Comparative Studies on the Effect of Tris -Egg Yolk and Soya Milk Based Semen Extender on various Semen Parameters for Effective Long Term Cryopreservation. Cardiometry; Issue 25; December 2022; p.1054-1061; DOI: 10.18137/cardiometry.2022.25.10541061; Available from: https://www.cardiometry.net/issues/no25-december-2022/comparative-studies-effect-tris-egg

Keywords

Semen,  Cryopreservation,  Novel semen extender,  Tris egg yolk extender,  Soya milk extender,  Assisted Reproductive Technology
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