Biological effects of mitochondrial therapy: preventing development of myocardial infarction and blocking metastatic aggression of B16/F10 melanoma

Aim

The aim is to evaluate the physiological parameters of the efficacy of cardiac mitochondria transplantation in male mice with chronic neurogenic pain and B16/F10 melanoma growth.

Materials and Methods

Male mice (n=37) of the C57BL/6 line were used in the research work. The animals covered experimental groups as follows: mice with chronic neurogenic pain (CNP) + B16/F10 melanoma (n=27); mice with CNB + B16/F10 melanoma + mitochondrial therapy (MC therapy) (n=10). Mitochondria were isolated from the heart of an intact rat with the use of differential centrifugation. An introduction of mitochondria to mice was carried out daily intraperitoneally at a dose of 3.3 mg of protein for 3 weeks. Statistical analysis of the results is carried out with the Statistica 10.0 software.

Results

On day 21 (week 3) of the experiment, macroscopically in the melanoma tissue in the group of animals with MC therapy, there were 2.5 times more necrosis cases than in the group without MC therapy. During the examination of the internal organs, no metastases were detected in the animals treated with MC therapy, while in 100% of the animals without MC therapy metastases were found in the lungs and in 95% of them in the spleen. In the animals received MC therapy, there was no damage to the heart muscle in 75% of the cases, while in the group of the animals without MC therapy, the presence of lesions in the form of bruises on the surface of the heart was macroscopically detected in 100% of the animals.

Conclusion

Thus, intraperitoneal transplantation of intact heart mitochondria contributed to the prevention of myocardial infarction and metastases to internal organs in the C57BL/6 male mice with B16/F10 melanoma growing against the background of chronic neurogenic pain.

Imprint

Oleg I. Kit, Elena M. Frantsiyants, Alla I. Shikhlyarova, Irina V. Neskubina, Irina V. Kaplieva, Natalia D. Cheryarina, Alexandra A. Vereskunova, Lidia K. Trepitaki, Yulia A. Pogorelova, Valeria A. Bandovkina, Ekaterina I. Surikova, Polina S. Kachesova, Elena A. Sheiko, Inga M. Kotieva, Marina A. Gusareva, Roza G. Luganskaya, Ekaterina S. Bosenko. Biological effects of mitochondrial therapy: preventing development of myocardial infarction and blocking metastatic aggression of B16/F10 melanoma. Cardiometry; Issue 22; May 2022; p.50-55; DOI: 10.18137/cardiometry.2022.22.5055; Available from: https://www.cardiometry.net/issues/no22-may-2022/biological-effects-mitochondrial-therapy