Mitochondrial therapy of melanoma B16/F10, pathophysiological parameters of tumor regression

Aim

The aim is to evaluate the pathophysiological parameters of the efficacy of liver mitochondrial transplantation in animals with B16/F10 melanoma.

Materials and Methods

In our experiment we used female and male mice of BALB/c Nude strain (n=28). Experimental groups were as follows: the reference group (n=14) with B16/F10 melanoma; the main group (n=14) with B16/F10 melanoma + mitochondrial therapy (MC therapy). Statistical analysis of results was carried out with the Statistica 10.0 software.

Results

The subcutaneous tumor in the mice of both sexes became detectable on day 5 from the time of the tumor inoculation, and the regressive effect produced by MC therapy was recorded in the males beginning with day 8 of the tumor growth. At the end of the experiment, on day 22, the difference in the average volumes of the tumor node was reported to be 3.2 times, i.e. a significant inhibition of the tumor growth in the group of the males with MC therapy was revealed. In the females on day 5 of the tumor growth, differences in the volume of the tumor focus between the reference group and the group with MC therapy were not recorded, however, a statistically significant difference was found in the sex-related comparison of the groups of the animals with MC therapy. It was determined that in the females with MC therapy, the area of the tumor spot during that period (5 days) was 1.4 times (p<0.05) less than that in the corresponding group of the males. On day 8, in the females completed MC therapy, the tumor has not yet concentrated into a solid structure, but remained as a flat tumor entity, and only by day 12 the tumor has formed from a flat structure into a volumetric tumor type. As a result, by the end of the experiment, on day 22, smaller volumes of the tumor nodes remained in the group of females treated with MC therapy, and the difference with the reference group was 2.7 times (p<0.05).

Conclusion

Thus, within the framework of the experiment, it has been found that the application of mitochondrial therapy using allogeneic liver mitochondria in the BALB/c Nude mice with B16/F10 melanoma retards the tumor growth in the mice of both sexes.

Imprint

Oleg I. Kit, Elena M. Frantsiyants, Alla I. Shikhlyarova, Irina V. Neskubina, Irina V. Kaplieva, Lidia K. Trepitaki, Yulia A. Pogorelova, Natalia D. Cheryarina, Alexandra A. Vereskunova, Valeria A. Bandovkina, Ekaterina I. Surikova, Natalia A. Maksimova, Inga M. Kotieva, Marina A. Gusareva, Viktoria V. Pozdnyakova. Mitochondrial therapy of melanoma B16/F10, pathophysiological parameters of tumor regression. Cardiometry; Issue 22; May 2022; p.56-61; DOI: 10.18137/cardiometry.2022.22.5661; Available from: https://www.cardiometry.net/issues/no22-may-2022/mitochondrial-therapy-melanoma-b16