BEST WASHING METHOD FOR OLIGOASTHENOSPERMIC PATIENTS BY EVALUATING DNA INTEGRITY BY USING A SPERM CHROMATIN DISPERSION TEST

Abstract

Background: Growing evidence points to sperm DNA fragmentation (DF) as a significant contributor to infertility. One of the most popular techniques to identify sperm DF is the Sperm Chromatin Dispersion test (SCDT), a new assay in semen. The SCDT is based on the idea that after acid denaturation and removal of nuclear proteins, sperm with fragmented DNA are unable to create the distinctive halo of dispersed DNA loops seen in sperm with non-fragmented DNA. As Oligoasthenospermic patients have low sperm concentration, these patients are infertile and opt for fertility treatments for getting a child. There are many steps in the processing of sperm during Assisted Reproductive Technology (ART), and one of the main steps is Sperm Washing. Aim & Objective: The main aim of this research is to determine the best sperm-washing method for Oligoasthenospermic patients by evaluating DNA integrity (DI) by using SCDT. Study Setting: Semen samples were obtained from 40 Oligoasthenospermic patients with progressive motility