Research methodology and methods used in experiment Mars-500 held at the Institute of Biomedical Problems of the Russian Academy of Sciences in 2009-2011 are considered. 6 volunteers were isolated during 520 days in a sealed ground-based facility simulating space ship.

It is shown that initiated by action of molecular viscosity impulse flow, directed usually from the moving fluid to limiting it solid surface, can, under certain conditions, turn to zero and get negative values in the case of non-stationary flow caused by alternating in time longitudinal (along the pipe axis) pressure gradient.

The paper presents the results of the long-term medical & ecological investigations conducted during the experiment Mars-500.

In order to evaluate the vegetative regulation of blood circulation and assess individual health risks under conditions of the 520 day isolation, used was an analysis of the heart rate variability (HRV).

The aim of the study was to evaluate the significance of cardioeigenoscopy method for the prognosis of risks of fatal arrhythmia (the primary and secondary VF) progression in AMI individuals.